Sub-Chronic Effects of Sodium Metabisulfite on the Intestinal Microarchitecture of Mice

Main Article Content

Sana Fatima
Hussain Khan
Muhammad Atif
Azka Zafar
Adil Farooq
Muhammad Fahad Baig
Asma Rashid Khan


Background: Sodium metabisulfite (SM) is commonly used with varying concentrations as a preservative, sewage treatment chemical, and in other commercial consumer products etc. It is reported to pose series of health hazards affecting various vital organs. The current study was aimed to find histological alterations in the intestine with sub-chronic exposure of SM in albino mice.

Materials and Methods: Ten mice were orally given SM (1.2 mg/kg mixed in 0.1 ml corn oil) for 21 days while ten mice were kept in the control group (no SM). All the mice were dissected, and small intestine pieces were excised and processed for histological sectioning and staining.

Results: SM disrupts the outer wall of villi and microvilli of the small intestine. Moreover, a narrow lumen and few mitotic figures were seen in the intestinal glands.

Conclusion: The results concludes that SM is a potential source of major organ’s defects and hence its usage should be lessened to improve the health status.

Article Details

How to Cite
Fatima, S., Khan, H., Majid, A., Atif, M., Zafar, A., Farooq, A., Baig, M. F., & Khan, A. (2022). Sub-Chronic Effects of Sodium Metabisulfite on the Intestinal Microarchitecture of Mice. Albus Scientia, 2022(2), 1–3.
Short Communications


Abbasi, M. H., Fatima, S., Khawar, M. B., Jahan, S., & Sheikh, N. (2018). An In Vivo Study on intoxicating effects of Nerium oleander water-based extract on Multiorgans of Wistar rat. Canadian Journal of Gastroenterology & Hepatology, 2018, 4739637. DOI:

Abbasi, M. H., Fatima, S., & Sheikh, N. (2014). Histological comparison of natural lung injury in Rattus norvegicus induced by a natural herb (Nerium oleander) and a known carcinogen (thioacetamide). Biologia, 60(1), 135–138.

Alimohammadi, A., Moosavy, M. H., Amin Doustvandi, M., Baradaran, B., Amini, M., Mokhtarzadeh, A., & de La Guardia, M. (2021). Sodium metabisulfite as a cytotoxic food additive induces apoptosis in HFFF2 cells. Food chemistry, 358, 129910. DOI:

Barberá, J., Metzger, A., & Wolf M. (2012). Sulfites, Thiosulfates, and Dithionites. Ullmann's Encyclopedia of Industrial Chemistry.

EFSA ANS Panel (EFSA Panel on Food Additives and Nutrient Sources Added to Food), 2016. Scientific Opinion on the re-evaluation sulfur dioxide (E 220), sodium sulfite (E 221), sodium bisulfite (E 222), sodium metabisulfite (E 223), potassium metabisulfite (E 224), calcium sulfite (E 226), calcium bisulfite (E 227) and potassium bisulfite (E 228) as food additives. EFSA Journal, 14(4): 4438 151. DOI:

Elmas, O., Aslan, M., Cağlar, S., Derin, N., Agar, A., Alicigüzel, Y., & Yargiçoğlu, P. (2005). The prooxidant effect of sodium metabisulfite in rat liver and kidney. Regulatory Toxicology and Pharmacology, 42(1), 77–82. DOI:

Naureen, I., Saleem, A., Nawaz, A., & Javed, M. (2021). Study the harmful effects of sodium metabisulfite on liver, testes, and lipid peroxidation in male mice, (Mus musculus). 2021. International Journal of Engineering Applied Sciences and Technology, 6(5), 30–38. DOI:

Noorafshan, A., Asadi-Golshan, R., Monjezi, S., & Karbalay-Doust, S. (2014). Sodium metabisulphite, a preservative agent, decreases the heart capillary volume and length, and curcumin, the main component of Curcuma longa, cannot protect it. Folia Biologica, 60(6), 275–280.

Vally, H., & Misso, N. L. (2012). Adverse reactions to the sulphite additives. Gastroenterology and Hepatology from Bed to Bench, 5(1), 16–23.

Yang, W. H., & Purchase, E. C. (1985). Adverse reactions to sulfites. Canadian Medical Association Journal, 133(9), 865–880.

Yoo, J., Lim, Y. M., Kim, H., Kim, E. J., Lee, D. H., Lee, B., Kim, P., Yu, S. D., Kim, H. M., Yoon, B. I., & Shim, I. (2018). Potentiation of Sodium metabisulfite toxicity by propylene glycol in both in vitro and in vivo systems. Frontiers in Pharmacology, 9,161. DOI:

Most read articles by the same author(s)